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Apr 14, 2011 · In contrast, cells expressing EGFP-S369X did not clearly exhibit fluorescence in the membrane, and the signal was mainly localized to the cytoplasm. Because S369X had no dominant-negative effect on WT in electrophysiological experiments, we then examined the interaction between WT and S369X . Cotransfection with non-GFP-tagged WT exhibited a ...

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